Germinal Biotech

GERMINAL BIOTECH

Protein Engineering

Yeast display

Yeast display is a technique for studying protein interactions and screening for proteins with high affinity to a particular target or high enzymatic activities against substrates. In this method, proteins are expressed on the surface of yeast cells and can be screened against various targets. Yeast display is particularly useful for antibody engineering, protein engineering, characterization of protein-protein interactions and enzyme evolution, offering the advantage of post-translational modifications that are more representative of eukaryotic systems.

We have established highly efficient workflow for yeast display, which can generate a library size of 5E8 in a single electroporation.

Protein Expression

Our comprehensive protein expression service caters to a wide range of research and industrial needs by offering protein production in bacterial, yeast and mammalian cell systems. Each system is tailored to suit different protein characteristics and applications, ensuring high-quality, efficient protein expression for various purposes.

Bacterial Expression

Utilizing E. coli, our bacterial expression system is ideal for cost-effective and rapid production of non-glycosylated proteins. This system is perfect for producing enzymes, antigens, and other proteins where post-translational modifications are not critical.

Yeast Expression

Our yeast expression platform, primarily using Saccharomyces cerevisiae, bridges the gap between prokaryotic and eukaryotic systems. It offers a balance of high yield and the ability to perform essential eukaryotic post-translational modifications. This system is particularly suited for producing recombinant proteins for vaccine development and industrial enzymes.

Mammalian Cell Expression

Our mammalian cell expression system, using cell lines like CHO, HEK293, and others, is the pinnacle of our service offerings for producing highly complex, glycosylated proteins. It is the system of choice for therapeutic proteins, antibodies, and other biologics, ensuring authentic post-translational modifications and bioactivity.

Cell Line Construction

Our cell line construction service employs both lentiviral and retroviral systems to efficiently deliver genes into a wide variety of cell types with high efficiency and ensure their long-term stable expression. Our services encompasses the design and production of custom viral vectors tailored to your specific needs, along with the transduction and selection of stable cell lines. 

Protein Expression

Co-detection by indEXing (CODEX) is a powerful tool for highly multiplexed, spatially resolved imaging of cells within tissue samples. Using fluorescence-labeled antibodies, CODEX sequentially images multiple biomarkers on a single sample, allowing for the detailed visualization of cellular interactions and microenvironments. This technique is particularly useful in studying complex biological systems such as tumors or inflamed tissues, where understanding the spatial arrangement and interaction of various cell types is critical.

Bacterial Expression

Utilizing E. coli, our bacterial expression system is ideal for cost-effective and rapid production of non-glycosylated proteins. This system is perfect for producing enzymes, antigens, and other proteins where post-translational modifications are not critical.

Yeast Expression

Our yeast expression platform, primarily using Saccharomyces cerevisiae, bridges the gap between prokaryotic and eukaryotic systems. It offers a balance of high yield and the ability to perform essential eukaryotic post-translational modifications. This system is particularly suited for producing recombinant proteins for vaccine development and industrial enzymes.

Mammalian Cell Expression

Our mammalian cell expression system, using cell lines like CHO, HEK293, and others, is the pinnacle of our service offerings for producing highly complex, glycosylated proteins. It is the system of choice for therapeutic proteins, antibodies, and other biologics, ensuring authentic post-translational modifications and bioactivity.