Case Studies

Three projects we can show the data for

These are the campaigns whose figures we are free to publish. They are a small part of the lab's project history — most of what we run stays confidential, and client identities are withheld throughout.

Therapeutic Lead Discovery

Hybridoma → BLI characterization

16 weeks, antigen to confirmed leads

  • Antigen design and production
  • Four-mouse immunization campaign
  • 20 plates screened by ELISA
  • 55 top hits confirmed by FACS and BLI
Bio-layer interferometry sensorgrams across five channels, association and dissociation phases fitted to a binding model.

Affinity Maturation Campaign

Yeast display → kinetics

8 weeks, parent clone to variants

  • Error-prone PCR library from the parent clone
  • Three rounds of MACS enrichment
  • FACS sorting for the highest-affinity variants
  • Top variants gained more than ten-fold in affinity
Flow cytometry dot plot overlaying the parental population with sort round two and sort round three, showing progressive enrichment.

Common Light Chain Screening

Existing LC → yeast display → kinetics

6 weeks, library to functional hits

  • Started from an existing light chain against antigen 1
  • Library built from mice immunized against antigen 2
  • Screened against antigen 2 on the shared light chain
  • Multiple functional antibodies confirmed
Blocking assay dose-response curves for the antibody against a negative and a positive control.

The rest of the project history

The three above are the ones with publishable data. The list below is what the lab actually spends its time on, across every service line, and it is a good deal longer than four projects.

DiscoveryFully human leads from transgenic mouse campaigns; VHH and scFv panels raised by phage display against targets that resist immunization; paired VH/VL panels from single-B cell sorting; multi-epitope panels screened for sandwich pairs.
EngineeringHumanization of rodent leads; developability repair on clones carrying sequence liabilities; engineering for species cross-reactivity; conversion between IgG, Fab, scFv and bispecific formats.
ProductionAntigen production for immunization and screening; bacterial constructs recovered from inclusion bodies by refolding; Fc fusions, multi-domain and glycosylated proteins in mammalian systems.
CharacterizationKinetics on clones supplied by the client; epitope binning across whole panels; HDX-MS epitope mapping; thermal stability and aggregation screening.
FunctionalReporter and stable overexpression lines built to spec; receptor blocking on cells; in vivo imaging in tumor-bearing models; PK/PD run alongside efficacy in the same study.
Ask about something closer to your problem. If none of the three matches what you are facing, describe it. We have very likely run something comparable and can talk it through in detail, under a confidentiality agreement where that is needed.

Have a similar problem?

If one of these looks like your situation, say so — it shortens the scoping conversation considerably.

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